Optimization of mouse embryo culture media using simplex methods

I-9: Important Components in Human Embryo Culture Media

Jacobsson AH Vitrolife Sweden AB, Goteborg, Sweden

Isolation and optimization of mice skeletal muscle satellite cells using preplating method and culture media substitution

Introduction: Satellite cells are known as the main regenerative cell type in skeletal muscles. Our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. Low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. M...

Effect of Two Polarized Culture System Prepared From Human Female Genital Tract on Mouse Embryo Development

Purpose: To compare effects of polarized epithelial monolayer prepared from human oviduct and uterus on mouse one and two-cell embryo development. Materials and Methods: Human oviduct and endometrial tissue was obtained from patients who had undergone total hysterectomy. The epithelial cells were isolated from tissue and cultured on extracellular matrix (ECM) Gel coated Millipore filter insert...

In Vitro Maturation, Fertilization and Embryo Culture of Oocytes Obtained from Vitrified Auto-Transplanted Mouse Ovary

Anther culture of iranian wheat germplasm using different media